Do you know where fluorescent proteins come from?

?

In recent years, the application of fluorescent proteins in the field of biological research has experienced explosive growth. In 1962, the green fluorescent protein (GFP) from?Aequorea victoria?(Victoria jellyfish) was discovered for the first time; it was successfully cloned in 1992 and used as a fluorescent label for in vivo labeling for the first time in 1994.

?

Subsequently, various GFP variants emerged. Since then, scientists have also successively identified fluorescent proteins from other species, which exhibit colors such as green, yellow, blue, and red. The spectral parameters of common fluorescent proteins are shown in the table below.?

?

Red Fluorescent Proteins and Their Antibodies

mCherry, also known as Cherry Fluorescent Protein, DsRed, or Red Fluorescent Protein, is a widely used red fluorescent tag protein, commonly applied in molecular labeling and the localization of cellular components, among other uses. Unlike GFP proteins isolated from jellyfish and other GFP variants, mCherry is a monomeric red fluorescent protein isolated from corals (Discosoma). It can be used in conjunction with GFP-series fluorescent proteins to achieve multicolor labeling. In vitro and in vivo experiments have shown that when mCherry is fused and expressed at the N-terminus or C-terminus of the target protein, there is no significant mutual impact between the activity of mCherry fluorescent protein and the function of the target protein. Therefore, mCherry is more popular than other fluorescent protein tags. In addition, mCherry has a unique advantage in viral labeling: researchers often use mCherry to label HIV to study the interaction between HIV and host cells, as well as the process of HIV infecting host cells. The red fluorescent protein tdTomato is a dimer formed by the tandem expression of two mCherry monomers, specifically designed to reduce protein aggregation. tdTomato exhibits stronger fluorescence than mCherry and has lower toxicity to cells/mice, etc., making it more commonly used in in vivo experiments.

The Anti-mCherry and Anti-tdTomato antibodies provided by SICGEN (distributed by XMJ) are both polyclonal antibodies targeting red fluorescent proteins extracted from?Discosoma?(disc corals). The antigens of these two antibodies (Anti-mCherry and Anti-tdTomato) are both purified from recombinant polypeptides expressed in?Escherichia coli?(E. coli). When Western blot is used to detect HEK293 cells transfected with the two proteins (mCherry and tdTomato), bands of 29 kDa and 55 kDa can be detected respectively. The Anti-tdTomato antibody exhibits specificity for both tdTomato and mCherry, but does not cross-react with green fluorescent?

?

Product Information

The red fluorescent protein antibodies from the SICGEN brand are suitable for a variety of immunological experiments. With excellent specificity, stability, and after-sales service, they have long been the brand’s star products and have received high praise from a wide range of customers. To facilitate your selection, we have compiled a product information table for SICGEN’s Anti-mCherry and Anti-tdTomato series antibodies.

SICGEN is a biotechnology company focusing on the development and production of antibodies for life science research. It provides high-quality and cost-effective polyclonal antibodies, as well as related products and services, to customers worldwide. The company is committed to discovering and developing new antibodies, and customizes polyclonal antibodies for top universities, research institutions, pharmaceutical enterprises, and biotechnology companies around the globe. It offers a full range of services from cDNA to affinity-purified antibody production, including antigen design, antibody synthesis, antigen conjugation, immunization, ELISA testing, purification, antibody labeling, and characterization. These efforts support innovative research in the field of life sciences.

?

Beijing XMJ?is the exclusive general agent of the SICGEN brand in China, providing customers with comprehensive technical support and after-sales service. Follow the official WeChat public account of "XMJ Technology" to learn more about "star products" in immunology. Beijing XMJ Technology Co., Ltd. has gathered a number of world-renowned suppliers of antibodies and antibody-related products and services, including SICGEN, Lifespan, MyBioSource, KPL, Nordic-MUbio, Exalpha, ProSpec, Everest, and Agrisera. With high-quality and a complete range of products, the company provides one-stop services for researchers in universities and research institutes, as well as biopharmaceutical production enterprises. If you are interested in the above-mentioned products, please call XMJ’s customer service hotline at 400-050-4006 or visit the official website?www.whuzsb.com?for inquiries and orders.

?

Statement: Some images in this article are sourced from the internet. If there is any infringement, please contact us to have them removed.

?